Hamilton MicroLab STAR
Automatic sample preparation system Hamilton MicroLab STAR, capable of autonomously processing up to 192 samples.
The system can open and close flip-tubes, perform temperature-controlled extractions, shake, centrifuge, purify using SPE plate technology, evaporate, and recover samples. These features greatly increase laboratory productivity (high-throughput) without compromising analytical performance.
Furthermore, thanks to a series of automatic checks designed to prevent incorrect volume dispensing, tip blockages, solvent depletion, and dripping, it is possible to work with microvolumes (µL scale) — a feature that not only reduces environmental impact but also allows for multiple extractions from very limited sample quantities.
Agilent 8890/7000E
Gas chromatograph coupled with a triple quadrupole mass spectrometer Agilent 8890/7000E, equipped with a dual multi-mode injector, a scan rate of 20,000 u/sec, MRM scan speed of 800 transitions/sec, MRM dwell time of 0.5 msec, EI MRM sensitivity <8 fg, and hydrogen as carrier gas.
The instrument is also equipped with a Gerstel MPS sample manipulator/injector for liquid analysis, an automated SPME Arrow sample preparation system, and a headspace sampler.
Its injection flexibility, scanning speed, and sensitivity make this system particularly suitable for the analysis of volatile (or derivatized) compounds through targeted or semi-targeted approaches in any matrix of interest.
Sciex QTRAP 6500+
Ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid quadrupole–collision cell–linear ion trap mass spectrometer Sciex QTRAP 6500+.
– MS/MS scan speed (quadrupole mode): 12,000 scan/sec
– MS/MS scan speed (trap mode): 20,000 scan/sec
– Polarity switching time in MRM: 5 ms
– Acquisition rate in MRM: 500 MRM/sec
– Sensitivity ESI+ <0.28 fg, ESI– <0.28 fg
The system’s ability to automatically switch within the same run between triple quadrupole and quadrupole/ion trap modes provides exceptional selectivity (reducing false positives). Combined with outstanding instrumental performance, this makes it particularly effective for targeted, semi-untargeted, and suspect screening analyses.
Sciex ZenoTOF 7600+
Ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid quadrupole–time-of-flight mass spectrometer (UHPLC-QTOF) Sciex ZenoTOF 7600+, equipped with an electron-activated dissociation (EAD) fragmentation system.
– MS scan speed: 25 scans/sec
– MS/MS DDA scan speed: 133 scans/sec
– MS/MS DIA (SWATH) scan speed: 200 scans/sec
– Mass accuracy ≤1 ppm
– Resolution: 40,000 FWHM at 956 m/z
The high-resolution time-of-flight technology, combined with the instrument’s scan speed, baseline stability, patented duty-cycle optimization, wide dynamic range, and stable LOD/LOQ, makes this system ideal for untargeted analyses of highly complex matrices. Additionally, the EAD system allows discrimination between isomeric compounds that cannot normally be distinguished using traditional fragmentation techniques (CID or HCD).
Thermo Orbitrap™ Ascend Tribrid™
Two-dimensional UHPLC system coupled with a hybrid quadrupole–linear ion trap–Orbitrap mass spectrometer (Thermo Scientific™ Orbitrap™ Ascend Tribrid™), equipped with ultraviolet photodissociation (UVPD) fragmentation.
– High-resolution MS scan speed: 45 scans/sec
– Low-resolution MS scan speed: 50 scans/sec
– Automatic polarity switching
– Resolution: 480,000 FWHM @ 200 m/z
The presence of 2D chromatography, high resolution, capability for MS¹⁰ experiments, inclusion of UVPD fragmentation, and the ability to acquire data simultaneously in high and low resolution (thanks to dual ion routing multipole IRM) make this instrument unique worldwide. It is essential for analyses where separation and characterization of isomeric or isobaric compounds are critical. Given its features, this system is particularly suited for proteomics analyses.
